Application

Anti-CHD-8, clone 7F4.1, Cat. No. MABN2265, is a highly specific mouse monoclonal antibody that targets Chromodomain-helicase-DNA-binding protein 8 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Research CategoryNeuroscience

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CHD-8 in human cervical cancer and human tonsil tissue sections.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Chromodomain-helicase-DNA-binding protein 8 (UniProt: Q9HCK8; also known as EC: 3.6.4.12, CHD-8, ATP-dependent helicase CHD8, Helicase with SNF2 domain 1) is encoded by the CHD8 (also known as HELSNF1, KIAA1564) gene (Gene ID: 57680) in human. CHD-8 is a member of the SNF2/RAD54 helicase family. Two isoforms of CHD-8 have been reported that are produced by alternative splicing. It is a DNA helicase that acts as a chromatin remodeling factor and regulates transcription. It also acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. CHD-8 is reported to suppress p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity and also acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin activity. It negatively regulates beta-catenin-targeted gene expression by being recruited specifically to the promoter regions of several beta-catenin responsive genes. CHD-8 is involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Mutations in CHD8 gene are associated with Autism 18 that is characterized by impairments in reciprocal social interaction and communication and developmental abnormalities.

Immunogen

GST/His-tagged recombinant fragment corresponding to 112 amino acids rom the C-terminal half of human Chromodomain-helicase-DNA-binding protein 8. The immunogen sequence is conserved in both isoforms.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CHD-8 in 10 µg of SH-SY5Y cell lysate.

Specificity

Clone 7F4.1 detects human Chromodomain-helicase-DNA-binding protein 8 (CHD8). It targets an epitope within 112 amino acids from the C-terminal half.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

270 and 300 kDa observed; 290.52 and 262.35 kDa calculated for isoforms 1 and 2, respectively. Uncharacterized bands may be observed in some lysate(s).